Rapid Differentiation of Mycobacterium tuberculosis and M. bovis by High-Resolution Melt Curve Analysis
نویسندگان
چکیده
منابع مشابه
Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis.
Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxy...
متن کاملRapid differentiation of Mycobacterium tuberculosis and M. bovis
45 46 Identification and characterization of the Mycobacterium tuberculosis strains is important for 47 clinical and therapeutic management of tuberculosis. Real time PCR with high-resolution melt 48 assay were found to improve the diagnostic process. The assay includes differentiation between 49 M. tuberculosis and M. bovis based on one SNP in the narGHJI and oxyR genes, and 50 determination o...
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Background: Pyrazinamide (PZA) is one of the most important drugs for tuberculosis (TB) treatment, however, its susceptibility is not routinely tested. High-resolution melting (HRM) curve analysis has been widely used for many applications. In this study, HRM assay was developed and evaluated for the detection of PZA resistance in Mycobacterium tuberculosis clinical isolates. Methods: Ninety fi...
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Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In this study, a multiplex PCR (mPCR) and high resolution melt (HRM) curve analysis were optimized for simultaneous detection and differentiation of C. jejuni and C. coli isolat...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00943-10